Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology
نویسندگان
چکیده
منابع مشابه
Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology
We previously demonstrated high-frequency, targeted DNA addition mediated by the homology-directed DNA repair pathway. This method uses a zinc-finger nuclease (ZFN) to create a site-specific double-strand break (DSB) that facilitates copying of genetic information into the chromosome from an exogenous donor molecule. Such donors typically contain two approximately 750 bp regions of chromosomal ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2010
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkq512